Background and Aim: COVID-19 is an acute respiratory disease, caused by a coronavirus named SARS-CoV-2. Previous reports suggest an association between COVID-19 infection and Hearing Loss (HL). We have investigated the Universal Newborn Hearing Screening (UNHS) results in the time period of COVID-19 epidemic in Guilan Province, north of Iran. Methods: We analyzed UNHS results from10 major obstetrics hospitals from 20th Mar 2020 to 19th Mar 2021, and compared the total UNHS positive/“, refer”,and total neonatal HL reports with the same time periods of recent three years. The prevalence of risk factors for HL were analyzed during the same period. Results: The total number of childbirth was decreased significantly from 27735 birth cases in Mar 2017-2018 to 18216 births in Mar 2020-2021 (p<0. 001), but the total “, refer”,rate was significantly increased from 63. 0 per 1, 000 births in Mar 2019-2020 to 79. 8 in Mar 2020-2021 (OR=1. 29, 95% CI: 1. 19-1. 39, p<0. 001), and also increased from 74. 7 per 1, 000 births in Mar 2017-2020 to 79. 8 in Mar 2020-2021 (OR=1. 07, 95% CI: 1. 01-1. 14, p=0. 020). The HL rate increased from 10. 3 per 10, 000 births in Mar 2019-2020 and from 11. 4 per 10, 000 births in Mar 2017-2020 to 18. 7 in Mar 2020-2021 (OR=1. 81 and 1. 63, 95% CI: 1. 06-3. 09 and 1. 10-2. 44, p=0. 028 and 0. 015, respectively). The prevalence of preterm birth (p=0. 039) and neonatal intensive care unit stay (p=0. 016) was increased significantly during Mar 2020-2021. Conclusion: The chance of “, refer”,results and neonatal HL, found in the UNHS program, increased during the COVID-19 epidemic in comparison to the past years.